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    Life Science

    Early stage embryoOur microscopy and life sciences solutions are designed to support applications from basic research to drug discovery to pre-clinical trials.

    Yokogawa’s high content analysis systems and dual spinning disk confocal technologies are used in regenerative medicine, drug discovery, and precision medicine, providing high-speed and high-resolution live cell imaging.

    • As the pioneer in dual spinning disk technology, Yokogawa's confocal scanner units enable real-time live cell imaging technology, transforming optical microscopes. 

    • Our high-content analysis (HCA) systems utilize powerful analysis software to address a range of research applications from basic science to drug discovery screening.

    We post our information to the following SNSs.
    Please follow us.

     

    Yokogawa Life Science

    •Twitter
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    Yokogawa's Official Social Media Account List

    Social Media Account List

    Principles of Spinning disk confocal

    The most common conventional confocal microscopes use a single laser beam to scan a specimen, while the CSU scans the field of view with approximately 1,000 laser beams, by using microlens-enhanced Nipkow-disk scanning: in short, CSU can scan 1,000 times faster.

    By using a disk containing microlens arrays in combination with the Nipkow disk, we have succeeded in dramatically improving the light efficiency and thus successfully made real-time confocal imaging of live cells possible.

    The expanded and collimated laser beam illuminates the upper disk containing about 20,000 microlenses (microlens array disk). Each microlens focuses the laser beam onto its corresponding pinhole, thus, effectively increasing laser intensity through pinholes placed in the pinhole array disk (Nipkow disk).

    With the microlens, backscattering of laser light at the surface of the pinhole disk can be significantly reduced, thus, dramatically increasing the signal to noise ratio (S/N) of confocal images.

    About 1,000 laser beams passing through each of the pinholes fill the aperture of the objective lens, and are then focused on the focal plane. Fluorescence generated from the specimen is captured by the objective lens and focused back onto the pinhole disk, transmitted through the same holes to eliminate out-of-focus signals, deflected by the dichroic mirror located between microlens array disk and the Nipkow disk to split fluorescence signal from reflected laser, passed through emission filter and then focused into the image plane in the eyepiece or camera.

    The microlens array disk and the Nipkow disk are physically fixed to each other and are rotated to scan the entire field of view at high speeds, thus, making it possible to view confocal fluorescent images in real-time through the eyepiece of the CSU head.

    As compared to conventional single point scanning, multi beam scanning by the CSU requires a significantly low level of light intensity per unit area, which results in significantly reduced photo bleaching and phototoxicity in live cells.

    Spinning Disk Confocal

    microlens / fastsacnning / minimal photo bleach / high resolution

    Microlens-enhanced Nipkow Disk Technology

    Microlens-enhanced Nipkow Disk Technology

    Comparison of scanning method

    point scanning

    Point Scanning
    1 line scan time=1[ms]
    1000 lines/image
    Scan lines=1000 [lines]
    1×1000=1000 [ms]

    disk scanning

    Disk Scanning by CSU
    Rotation Speed=10000 [rpm]=41.7[rps]
    30°Rotation/image
    1÷( 41.7×30/360 )= 0.5 [ms]

     

     

    January 20,2020

    Sales release : High Content Analysis Software CellPathfinder update and Deep learning option was released. 

    Deep learning function succeeded in dramatically improving by recognizing patterns in images to identify what the images show.

    Link to products High Content Analysis Software CellPathfinder
    Find out more about Deep learning

    January 15,2020

    Society for Laboratory Automation and Screening (SLAS) 2020

    January 25-29, 2020
    Booth No. 1148

    We will exhibit high content analysis system "CellVoyager CQ1".

    Link to products
    Confocal Quantitative Image Cytometer CQ1

    -Poster-

    1207-C:
    A Live Cell High Content Assay for Cellular Lipid Droplets
    January 28, 2:00-3:00 pm

    December 6,2019

    Sales release : A flat-top beam shaper option CSU-W1 Uniformizer

     Click Here For More Info

    November 18,2019

    ASCB/EMBO 2019

    December 8-10, 2019
    Booth No. 300

    We will exhibit Spinning disk confocal "CSU-W1 SoRa" and high content analysis system "CellVoyager CQ1".

    Link to products
    Super Resolution Confocal Scanner Unit : CSU-W1 SoRa
    Confocal Scanner Unit : CSU-W1
    Confocal Quantitative Image Cytometer CQ1

    -Tech talks-

    December 9, 3:00-4:00 pm – Theater 2, Learning Center

    Uniformizer, a new flat-top beam shaper for CSU-W1 & Introduction to new approach for single cell analysis

    Presenter: Naoki Ando: Product specialist of CSU, Masahiro Kajita: Project manager of “Single Cellome”

    Yokogawa continues to make improvements to provide the best products for biology researchers. So far, CSU has fulfilled the researchers' wish to take images fast, wide, clear and in super-resolution. This time, we will introduce the new option “Uniformizer” of our product “CSU-W1”. This unit uniformizes the laser illumination and enables quantitative imaging. This provides a large seamless montage image between each field. Furthermore, Yokogawa also takes new challenge for the next field, which is single cell analysis. As a trial, it had prototyped automated single cell sampling system, and it has possible to investigate the molecular characterization of each single cell. This tech talk will introduce 1) ”Uniformizer” and 2) new approach for single cell analysis, which could perform with confocal microscopy.

    October 3,2019

    SLAS 2019 Advanced 3D Human Models and High-Content Analysis Symposium

    October 21-12, 2019
    The Francis Crick Institute, London, United Kingdom
    Booth No. 5

    We will exhibit high content analysis system "CellVoyager CQ1".

    Link to informations
    Confocal Quantitative Image Cytometer CellVoyager CQ1

    April 8,2019

    FOM 2019

    April 14-17, 2019
    Queen Elizabeth II Center
    Booth No. 42

    We will exhibit Spinning disk confocal "CSU-W1 SoRa".

    Link to products
    Super Resolution Confocal Scanner Unit : CSU-W1 SoRa
    Confocal Scanner Unit : CSU-W1

    January 16,2019

    SLAS 2019

    February 4-6, 2019
    Walter E. Washington Convention Center
    Booth No. 214

    We will exhibit high content analysis system "CellVoyager".

    Link to products
    CellVoyagerTMCV8000 High-throughput Cytological Discovery System
    Confocal Quantitative Image Cytometer CQ1
    High Content Analysis Software CellPathfinder

    *Poster presentation is planned. Details will be posted as soon as it is decided.

    October 24,2018

    ASCB/EMBO 2018

    December 9-11, 2018
    Booth No. 845

    -Tech talks-

    December 9, 3:00-4:00 pm – Theater 2, Learning Center

    Super Resolution Confocal Scanner Unit CSU-W1 Sora

    Presenter: Takuya Azuma: Chief designer of CSU-W1 Sora,
    Yoshitaka Sekizawa: Product manager of CSU-W1 Sora

    Yokogawa will introduce our brand-new product “CSU-W1 SoRa.” This is a spinning disk based super resolution confocal scanner unit. In this talk, we will introduce features and principles of this product and we will show beautiful image samples taken by “CSU-W1 SoRa”. Features of “CSU-W1 SoRa”: 1) XY resolution of approx. 120nm. XY resolution has been improved by approximately 1.4x the optical limit based on spinning-disk confocal technology. Furthermore, a final resolution approximately twice the optical limit is realized through deconvolution. 2) Ideal for super-resolution live cell imaging. Just like the CSU, high-speed real time imaging can be performed with super-resolution. In addition, live cell imaging is possible, reducing bleaching and phototoxicity. 3) The CSU is easy to use. Super-resolution images can be observed in real time without any specific preparation of sample. Deep position observation is made possible through optical sectioning based on confocal technology. 4) Upgradable from CSU-W1. If you already have CSU-W1, you can add SoRa disk.

    September 14,2018

    Sales release : High Content Analysis Software CellPathfinder

     Click Here For More Info

    July 27,2018

    Sales release : High-speed Super resolution Confocal Scanner CSU-W1 SoRa

     Click Here For More Info

    June 11,2018

    2018 SLAS Europe

    March 01,2018

    Sales release : High Content Data Management System CellLibrarian

     Click Here For More Info

    December 29,2017

    SLAS 2018

    February 3-7, 2018

    December 29,2017 Sales news : The Discontinuation of CellVoyagerTM CV7000S High-throughput Cytological Discovery System
    September 05,2017

    Sales release : CellVoyagerTMCV8000 High-throughput Cytological Discovery System

     Click Here For More Info

    January 19,2017

    SLAS High-Content Screening Conference 2017

    April 04,2016

    Poster presentation in 3D Cell Culture 2016, 19-21 April 2016, Konzerthaus Freiburg/Germany

    Yokogawa Electric Corporation will present data obtained by our confocal image cytometer CQ1 in “3D Cell Culture 2016: How close to ‘in vivo’ can we get? Models, Application & Translation”. The poster will show the results of 3D live cell imaging and analysis of the migration and the network formation of HUVEC cells in a multilayered cell sheet. The results demonstrate that CQ1 is an excellent research tool in the field such as regenerative medicine and drug discovery screening.

    *Data were provided from BioProcess Systems Engineering Lab., Dept.Biotech., Grad. Sch. Eng., Osaka University.

    February 10,2016 Yokogawa Concludes Distribution Agreement with Optec, LLC for Sale of Confocal Quantitative Image Cytometer CQ1 at the markets of OPTEC activity
    October 01,2015 Sales release : Label-free Morphological Analysis Software CellActivision

     

    Overview:

    Visualizing the cell behavioral basis of epithelial morphogenesis and epithelial cancer progression

    Overview:

    Faster, Deeper, and Clearer -in vivo molecular imaging technology-

    Overview:

    Discovering the Basic Principles of Life through the Live Imaging of C. elegans

    Overview:

    Use of the spinning disk confocal at the Harvard Medical School microscopy core.

    Overview:

    Spinning Disk Confocal Microscopy for Quantitative Imaging and Multi-Point Fluorescence Fluctuation Spectroscopy.

    Overview:

    New Era in Manmmalian Genetics Research: To utilize the same embryo after long-time 3D observation!

    Overview:

    On-site manipulation of protein activities: Understanding intricate cell signaling pathways.

    Overview:

    Getting Closer to “Plant Cell World”with High-speed Live Imaging and Image Information Processing.

    Overview:

    Closing in on Neuronal Circuit Dynamics through High-speed, fMCI.

    Overview:

    To investigate interactive dynamics of the intracellular structures and organelles in the stomatal movement through live imaging technique, a CSU system was used to capture 3-dimensional images (XYZN) and time-laps images (XYT) of guard cells.

    Application Note
    Application Note
    Application Note
    Application Note
    Overview:

    The CQ1 confocal image acquisition mechanism with the distinctive CSU® unit has a function to sequentially acquire fine cell images along the Z-axis and capture information from the entire thickness of
    cells which include heterogenic populations of various cell cycle stages. In addition, saved digital images can be useful for precise observation and analysis of spatial distribution of intracellular molecules.
    The CQ1 capability to seamlessly analyze images and obtain data for things such as cell population statistics to individual cell morphology will provide benefits for both basic research and drug discovery
    targetingM-cell cycle phase.

    Application Note
    Overview:

    Cell clusters are directly measured with high-throughput 3D imaging
    Confocal Quantitative Image Cytometer

    Application Note
    Overview:

    Wide and Clear
    Confocal Scanner Unit

    Application Note
    Overview:

    CV1000 clears the hurdle in Live Cell Imaging
    All-in-one Live cell imaging solution

    Overview:
    • Colony Formation
    • Scratch Wound
    • Cytotoxicity
    • Neurite Outgrowth
    • Co-culture Analysis
    • Cell Tracking
    Overview:

    Faster, Brighter, and More Versatile
    Confocal Scanner Unit

    Application Note
    Overview:

    Welcome to The New World of High Content Analysis
    High-throughput Cytological Discovery System

    Yokogawa Technical Report
    The World as Seen from Cells
    (rd-te-r06002-001)
    2.2 MB
    Yokogawa Technical Report
    6.1 MB
    Overview:

    This "Tutorial" provides overview of this software, from installation through data analysis.

    Overview:

    In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

    Overview:

    In this tutorial, a method for analyzing ramified structure, using CellPathfinder, for the analysis of the vascular endothelial cell angiogenesis function will be explained.

    Overview:

    In this tutorial, spheroid diameter and cell (nuclei) count within the spheroid will be analyzed.

    Overview:

    In this tutorial, we will learn how to perform time-lapse analysis of objects with little movement using CellPathfinder, through calcium imaging of iPS cell-derived cardiomyocytes.

    Overview:

    In this tutorial, we will identify the cell cycles G1-phase, G2/M-phase, etc. using the intranuclear DNA content.

    Overview:

    In this tutorial, image analysis of collapsing stress fibers will be performed, and concentration-dependence curves will be drawn for quantitative evaluation.

    Overview:

    In this tutorial, we will observe the change in number and length of neurites due to nerve growth factor (NGF) stimulation in PC12 cells.

    Overview:

    In this tutorial, intranuclear and intracytoplasmic NFκB will be measured and their ratios calculated, and a dose-response curve will be created.

    Overview:

    In this tutorial, we will learn how to perform cell tracking with CellPathfinder through the analysis of test images.

    Overview:

    In this tutorial, using images of zebrafish whose blood vessels are labeled with EGFP, tiling of the images and recognition of blood vessels within an arbitrary region will be explained.

    Looking for more information on our people, technology and solutions?


    Contact Us
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